Chromatography: How can we separate a mixture?

PurposeThe chromatography lab is to understand how molecules with standardised molecular properties can be scattered with story chromatography. These differences will be interpreted to see the distinction of separate chemical capacitys.Pre laboratory Questions1. apologise capillary action as it pertains to water and paper. capillary action makes water lay down up the paper. As paper absorbs water mixes with the solvents in the paper.2. What is the Rf prise in a chromatography experiment?Rf = Distance travelled by the solute from the pilot film profligate/distance travelled by the solvent from the original line3. If a molecule has a postgraduate affinity for the stationary phase, how is the Rf evaluate affected?High affinity for the stationary phase affects the Rf repute by demean Rf values.4. If a molecule has high affinity for the mobile phase, how is the Rf value affected?The Rf value will be higher5. Imagine you are doing a chromatography experiment with a polar sol vent and a molecule containing a carbonyl group. Would the Rf value be high or low? Explain.The Rf value would be predicted as organism low because it would tend to stick to the paper more.6. Why must a pencil be used, instead of a pen or marker when marking chromatography exfoliations?A pencil is being used when parking chromatography plates because the ink could take part in reacting with the substance that it is placed in.7. Why should latex paint gloves be worn when preparing chromatography plates?Latex gloves should be worn to prevent contamination of the chromatogram8. The sample fine thin-layer chromatography plate, shown downstairs, was prepared by spotting methyl red at R, sudan III at S, and bromocresol kelvin at G. A single drop of all(prenominal) was placed on M. The plate was put in the evolution solution until the solvent bet r from each oneed 10 cm. Estimate the retention factor of R,S, and G, by measuring to the centre of the spot.0.625 .369. Describe how th e TLC plate shown in question 8 was im the right way prepared. For thin layer chromatography the adsorbent is coated as a thin layer onto a suitable support. This layer substance variety show is separated by elution with a suitable solvent.10. Suppose that, while one of the chromatography plates is developing, the beaker is accidently bumped, and the developing solution splashes on the TLC plate. Explain how this would influence the results.The results would shift dramatically towards the selected solution before.Materials ListFood tint solutions, 3 colors and an unknown region discolor mixture Sodium chloride solution Isopropyl alcohol Paper chromatography plates Capillary tubes Pencil Five 250-mL beakers Plastic wrap Metric ruler Lab notebook Latex gloves, safety goggles, lab apronsProcedure Activity 11. Wearing latex gloves, obtain ten chromatography plates, as directed by the instructor. Prepare each chromatography plate by marking lightly with pencil, a line at the bottom. Draw two small dots on the bottom line. Place the labels B (blue) and R (red) below the dots on the line. Repeat with the yellow fodder dye (Y) on another chromatography paper. Prepare the remaining eight plates the same federal agency so that you have five sets of chromatography plates. 2. Properly prepare 250 mL beakers3. rear 250 mL beakers with plastic wrap 4. Prepare 10 mL of below solution and place them in the beakers. Label with the mobile phase composition. a. 1% salinity water b. 1% salt water/isopropyl alcohol (31) mixture c. 1% salt water/isopropyl alcohol (11) mixture d. 1% salt water/isopropyl alcohol (13) mixture e. isopropyl alcohol 5. cover each beaker with a piece of plastic wrap 6. Prepare 1 mL of each dye solution 7. Place one drop of blue food dye with the capillary tube 8. Do this for the R (separate capillary tube) 9. Do this for the Y (separate capillary tube) 10. Allow droplet to fully alter 11. Gently lower one of the plates into one of the 250-mL devel oping solution beakers, ensuring that the dry food dye spots are at the bottom.Ensure thatno solvent splashes onto the chromatography plate above the initial solvent aim 12. Carefully re-cover the 250-mL beaker13. The solvent will chop-chop rise through the plate. Allow until way 14. Once the solvent level has reached the maximum height, quickly remove the plate from the 250-mL beaker and mark the exact agitate of the solvent front before the solvent evaporates. (will take longer) 15. Mark the plate with the identity of the developing solution composition. Set the plate face-up to allow it to dry 16. Replace the plastic wrap cover on the 250-mL beaker17. Repeat stairs 7-16 with each of the other developing solutions. Ensure that each plate is properly marked and labeled 18. Measure the distance between the bottom line and the upper solvent line on each plate. Record this info in the entropy table 1 for the corresponding developing solution 19. Identify and circle each spot co rresponding to the indicator dyes on each of the chromatography plates 20. Measure the distance between the bottom line and the tenderness of each indicator spot (B,R andY) on each plate. Record this data in the corresponding data table 21. Ask the instructor whether the chromatography plates should be retained or disposed off